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First ‘One-Health’ study on genome-wide comparison of multidrug-resistant Escherichia coli from Human-Animal-Aquaculture-Environment continuum: a collective effort from six institutes of India
  • +11
  • B Shome,
  • Rakshit Ojha,
  • Suresh Mendem,
  • Devi Murugesan,
  • Gopalakrishna Sivaraman,
  • Sudha Sajeev,
  • Sandeep Ghatak,
  • Chimanjita Phukan,
  • Suranjana Chaliha Hazarika,
  • Zunjar Dubal,
  • Shaikh Ziauddin Ahammad,
  • Rajeswari Shome,
  • Claire Raisen,
  • Mark Holmes
B Shome
National Institute of Veterinary Epidemiology and Disease Informatics
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Rakshit Ojha
National Institute of Veterinary Epidemiology and Disease Informatics
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Suresh Mendem
National Institute of Veterinary Epidemiology and Disease Informatics
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Devi Murugesan
National Institute of Veterinary Epidemiology and Disease Informatics
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Gopalakrishna Sivaraman
Central Institute of Fisheries Technology
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Sudha Sajeev
Central Institute of Fisheries Technology
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Sandeep Ghatak
ICAR Research Complex for North Eastern Hill Region
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Chimanjita Phukan
Gauhati Medical College and Hospital
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Suranjana Chaliha Hazarika
Gauhati Medical College and Hospital
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Zunjar Dubal
Indian Veterinary Research Institute
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Shaikh Ziauddin Ahammad
Indian Institute of Technology Delhi
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Rajeswari Shome
National Institute of Veterinary Epidemiology and Disease Informatics
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Claire Raisen
University of Cambridge Department of Veterinary Medicine
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Mark Holmes
University of Cambridge Department of Veterinary Medicine
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Abstract

Summary: Antibiotic-resistant bacteria are emerging threat to the global public health. Estimates suggests >10 million deaths by 2050 due to AMR alone. Six Indian institute collaborated along with 4 UK universities to address the issue of antimicrobial resistance (AMR) through One Health approach under NEOSTAR project jointly funded by Department of Biotechnology (DBT), Government of India and UK Research and Innovation (UKRI), United Kingdom. To address a question “Dose AMR in livestock contributes to AMR in people? A pilot study was conducted which is a collaborative effort to collect archival microbial isolates of Escherichia coli, Klebsiella spp., Staphylococcus aureus and coagulase negative S. aureus (CoNS). The current study deals with E. coli isolates collected from ICAR-NIVEDI, ICAR-CIFT, ICAR-IVRI, ICAR-RCNEH, IIT-Delhi and GMCH and were subjected to QC analysis at ICAR-NIVEDI’s BSL2+ facility, these isolates were further segregated into 5 components viz., Animal origin (including aquaculture) (n=50) food of animal origin (n=30), Human (community settings) (n=3), Hospital origin (n=15) and Environment origin (n=14). A total of 112 E. coli isolates sequenced for whole genome through Illumina’s HiSeq 2500 platform at Sangers Institute, UK and paired end libraries were generated for all the 112 isolates were assembled and subjected to genome characterization and gene mining through various bioinformatics tools revealed the frequency of resistance, co-resistance, and resistant genes are high and similar across Human-Animal-Aquaculture-Environment continuum in India. This emphasizes the need to collaborate and mitigate antibiotic resistance with a ‘One Health’ approach.