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High efficiency preparation of standard grade γ-tocopherol in plant-scale and its antioxidant activity
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  • Yaping Li,
  • Linshang Zhang,
  • Zhide Wang,
  • Yanlan Bi,
  • Dan Peng
Yaping Li

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Linshang Zhang
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Zhide Wang
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Yanlan Bi
Henan University of Technology
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Natural standard grade γ-tocopherol are of great interest due to its good antioxidant activity and special pharmacological functions. This study separated and purified γ-tocopherol from mixed tocopherols using eco-friendly and low toxicity solvents. 0.28 g γ-tocopherol was obtained per performance with a purity of 98.89 ± 0.68% and a recovery yield of 93.23 ± 0.89%. The purification conditions were as follows: elution solvent n-hexane/ethyl acetate 94.5:5.5 (v/v), sample load size 0.5 g, a column height to diameter ratio of 16:1 and an elution rate of 2 mL/min. The purity and structure of γ-tocopherol was confirmed by HPLC, GC/MS and NMR. The adsorption isotherm of γ-tocopherol on silica gel (200-300 mesh) fitted the Langmuir isotherm model well. The antioxidant activity analysis showed that γ-tocopherol had the strongest antioxidant activity followed by δ-tocopherol and α-tocopherol. This study provides an economically attractive solution for the production of natural standard grade γ-tocopherol.