BDNF Val66Met was associated with the susceptibility of PE but
irrespective of neonatal poor prognosis in a Han population：a
Objective: Brain-Derived Neurotrophic Factor (BDNF) plays a role in
placental development and is involved in the pathogenesis of
preeclampsia (PE). In this study, we aimed to investigate the
correlation of Val66Met variation in BDNF and PE and further explore the
possible relationship between Val66Met and neonatal poor prognosis.
Methods: TaqMan probe fluorescent PCR was used to analyze the genotypic
and allelic frequency of BDNF Val66Met in 1138 cases of the PE group and
1342 cases of the control group. Besides, 200 pairs pregnant women with
PE and their newborn, along with 208 pairs healthy women and their
newborn were enrolled to evaluate the mother-fetal transmission effect.
Furthermore, we detected the expression level of BDNF in placental
tissue at the RNA and protein levels in 21 PE patients and 21 healthy
pregnant women. Results: We found that the allele distribution was
significantly difference in case group and control group (2=4.657,
P=0.031, OR=0.884, 95%CI=0.791-0.989), suggesting BDNF Val66Met may be
related to PE susceptibility. While the genotype distribution and
additive gene have no significant difference. The analysis of
maternal-fetal pairing showed the transitivity of BDNF Val66Met have no
significant difference between the case group and the control group.
Besides, the mRNA and protein expression level of BDNF in PE group was
significantly lower than that in control group. Conclusion: We found
that BDNF Val66Met may be associated with the occurrence of PE, and
allele G may play a protective role. However, this locus may be not
related to the poor neonatal prognosis.