Although sex assignment is essential to study biology and ecology of a
given animal, in Xenarthra there is still no standardized assay for
gender genetic identification. Here, we evaluate the potential of two
nuclear fragments [SRY (~180pb) and Zinc finger
(~400pb) genes] for sex identification of specimens
who have lost their morphological characteristics because of road-kills.
DNA samples from seven Xenarthra species were amplified and sequenced
for one or both segments. Finally, we performed a case study using
tissue samples from road-kill carcasses, which supported the suitability
of our markers for poor-quality DNA. The method proved to be efficient
for different types of samples and may be especially useful for studies
using road-killed and hunted animals.