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Complete genome and sequence and phylogenetic analyses of the first bovine norovirus strain identified in northern China
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  • Meng-Jia Wang,
  • Jinghui Fan,
  • Hui-Xia Fan,
  • Ying Liu,
  • Zhi-Hao Peng,
  • Yu-Zhong Ma,
  • Qing-Yan Li,
  • Yu-Zhu Zuo
Meng-Jia Wang

Corresponding Author:[email protected]

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Jinghui Fan
Hebei Agricultural University
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Hui-Xia Fan
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Zhi-Hao Peng
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Yu-Zhong Ma
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Qing-Yan Li
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Yu-Zhu Zuo
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Bovine norovirus (BNoV) is a recently identified calicivirus in the genus Norovirus, which can cause enteric disease with clinical signs including vomiting, diarrhea, dehydration, and mortality in neonatal calves. BNoV has been reported in different countries all over the world. We collected 39 fecal, blood, and tissue samples from calves with diarrhea from Hebei in northern China in 2019. The presence of BNoV was determined by PCR only two samples (5.1%) were positive. The complete genome of the newly identified strain Bo/CH/HB/BD/2019 was successfully sequenced (GenBank accession number: MN480761). Based on the existing gene sequences in the GenBank database, evolutionary trees were constructed. Sequence analysis showed that the nucleotide sequence homology of the Bo/CH/HB/BD/2019 genome with BNoV genomes in GenBank was 84.0–92.4%. Bo/CH/HB/BD/2019 has a GIII.2 genotype, and further analysis of its VP1 gene revealed four amino acid substitutions, i.e., 225C, 246T, 624T, and 945T. To the best of our knowledge, this is the first time the complete genome of a BNoV isolate from northern China has been sequenced.