SARS-CoV-2 variants with spike (S)-protein D614G mutations now predominate globally and increase infectivity by assembling more functional S protein into the virion. In this paper, I combine topology geometric methods for the generation of novel drug designs by using generalized k - nearest neighbors within a quantum computing chemical context targeting in a atomistic level the S proteins with aspartic acid (SD614) and glycine (SG614) at residue 614 protein apparatus. In this effort, I propose powerful enough computer - aided rational drug design strategies to achieve very high accuracy levels for the generation of AI - Quantum designed molecules of GisitorviffirnaTM, Roccustyrna_ gs1_TM, and Roccustyrna_fr1_TM ligands targeting the SARS - COV - 2 SPIKE D614G mutation by unifying Eigenvalue Statements into Shannon entropy quantities as composed on Tipping–Ogilvie driven Machine Learning potentials for nonzero Christoffel symbols. For this model, I find analytic black hole solutions relevant to address a vast variety of small molecule modeling problems essential to describe pharmacophore merging phenomena in the presence of chemical potentials among others at the locally AdS5 spacetime. A boundary solution in five-dimensional Chern-Simons supergravity is described in the form of a Quantum Circuit which can carry U(1) charge provided the spacetime torsion is non-vanishing. Thus, I analyze the most general configuration consistent with the local AdS5 generated D614G Binding Site isometries in Riemann-Cartan space. I also arrived at a new Zmatter derived finite ‐ dimensional state integral and a Schwarzschild (DFT) ℓneuron (ι) : = φ∘D∘r2∘S∘r102 /3[T] Ψ0⋮Ψ0Ψ0e [r] (F ∧ F ∧ F) (1o∑ ∑ ∑ ) improver for a Chern - Simons Topology and Euclidean symplectic ω = (i~)− 1/2 F (ab)J(ab) o1 ℓ T (a)J(a) oF T1 (dx/x) ∧ (dy/y) model by computing the analytically continued “holomorphic blocks” on an appropriate quantum Hilbert space H to put pharmacophoric elements back together.

To our knowledge this is the first published case of NP associated with COVID-19 in an individual with CF and the first associated with Nocardia infection. We suspect the combination of cystic fibrosis, COVID-19 pneumonitis and co-infection with Nocardia farcinia caused this young man’s NP and ultimately his untimely death. We hope this case will highlight individuals with CF of all ages are at risk of severe COVID-19 infection.

Background This study aims to described the epidemiology and genotypic diversity of Human metapneumovirus (HMPV) and the impact of SARS-CoV-2 on the prevalence of HMPV in hospitalized children with Acute respiratory tract infections (ARTIs) in Beijing, China. Methods From April 2018 to March 2019 and from September 2020 to August 2021, nasopharyngeal aspirates (NPAs) from hospitalized children with ARTIs in Beijing were collected and subjected to real-time polymerase chain reaction tests for HMPV. Then genotyping, detection of 15 common respiratory viruses and clinical characteristics were analyzed on HMPV positive samples. Results 7.9% (124/1572) enrolled paediatric patients were identified as having HMPV infection, and the majority of children under the age of 5 (78.2%, 92/124), From April 2018 to March 2019. The detection rate of HMPV in spring and winter is significantly higher than that in summer and autumn. The co-infection rate were 37.1% (46/124), the most common co-infected virus were parainfluenza virus type 3 (HPIV-3). The main diagnosis of HMPV infection was pneumonia (92.7%,115/124), most patient have cough and fever. Of 78 HMPV-positive specimens, A2b (82.1%,64/78) were the main epidemic subtypes. .During the COVID-19 outbreak, Among 232 samples, only 4 cases were HMPV-positive. After statistical test, the detection rate of HMPV during the COVID-19 pandemic has decreased significantly compared with that before the epidemic (p=0.001). Conclusions HMPV is an important cause of ARTIs in children under 5 years old. Under the prevention and control of the COVID-19 pandemic, the HMPV infection of hospitalized children with ARTIs has decreased significantly.

General methods to quantize combined descriptors of both the probability and phase/current distributions in molecular electronic reference frame transformations, to a “superposition of coordinate transformations” have been previously introduced. Continuity equations for these densities are explored on an array of recent fundamental observations developed through gravitational amplification of primeval density fluctuations generated in the exceedingly early phase of cosmic evolution. In this paper, I show that the notion of vertical-equilibrium state of the vanishing current entanglement in chemical space and orbital superpositions are observer-dependent features in quantum reference frames including Galilean transformation, and near-horizon symmetries ranging from supergravity theories to Lorentzian cryptographic signatures to enhance the RoccuffirnaTM’s gravity to trap the SARS-COV-2 viruses in practice. The corresponding stationary radial Schrödinger equations with these potential energy functions are solved analytically, in the underlying information continuity equation that determines machine learning characteristics for both Euclidean and Lorentzian signatures to Quantum microBlackHole-Inspired Kerr- (A) dS-Myers–Perry black Gravitationals in an approximate scheme for zero total angular momentum. It is found that the wave functions for bound states can be expressed in terms of the Jacoby polynomial involving either the nonclassical or resultant entropy/information concepts the so called horizontal-equilibrium state, which represents the phase-transformed quantum state of a molecule, corresponding to the optimum, density-dependent “thermodynamic” phase in the near-horizon limit, with either SL(2, ℝ) or Poincaré iso(1, 1) symmetry which extends to the entire near-horizon geometry . This analytical expressions for purely vibrational energy levels of the electronic state, without an accompanying change of quantum observables, preserves the electron probability distribution but modifies its current by varying the moduli to a weakly-coupled description of a pharmacophoric system, where states with fixed conserved charges can be counted. The influence of the equilibrium-phase gravitational transformation, quantum phase density, and local entropy formalism production is examined on the continuity chemical relations for the anti-COVID-19 RoccuffirnaTM molecular equilibrium states.

Our patient is a 39-year-old male with normal baseline blood parameters who presented with COVID-19 infection, associated with neutropenia and then progressed to critical disease culminating into CSS. Based on risks and benefits evaluation, he was treated with Tocilizumab reinforced with G-CSF leading to full recovery including reversal of neutropenia.

Background. Factors related to an adverse evolution in COVID19 infection are needed for proper decision making. We try to identify factors related to hospitalization, ICU admission, and mortality related to the infection. Methods. Retrospective cohort study of patients with SARS-CoV-2 infection from March 1st 2020 to January 9th 2022. The sample was randomly divided into two subsamples, for the purposes of derivation and validation of the prediction rule, until omicron variant appearance and afterwards, respectively. Data collected for this study included sociodemographic data, baseline comorbidities and treatments, and other background data. Multivariable logistic regression models using Lasso logistic regression were used . Results. In the multivariable models, older age, male, peripheral vascular disease, heart failure, heart disease, cerebrovascular, dementia, liver, kidney, diabetes, hemiplegia, interstitial pulmonary disease, cystic fibrosis, malignant tumors, as well as diuretics and the chronic systemic use of steroids were common predictive factors of death. Similar predictors, except liver disease, plus arterial hypertension, were also related to adverse evolution. Similar predictors to the previous, including liver disease, plus dyslipidemia, inflammatory bowel disease, respiratory diseases, and the basal prescription of NSAIDs, heparin, bronchodilators, or immunosuppressants were related to hospital admission. All risk scores developed had AUCs from 0.79 (hospital admission) to 0.94 (death) in the validation in the omicron sample. Conclusions. We propose three risk scales for adverse outcomes and hospital admission easy to calculate and with high predictive capacity, which also work with the current omicron variant, which can help manage patients in primary, emergency, and hospital care.

Non covalent biliproteins are found in a growing number of living organisms and even in viruses, such as SARS-CoV-2. Unlike the well described covalent biliproteins, such as the phytochromes, they present a vast structural and functional diversity, and often with limited experimental information. A very important tool (and sometimes the only one available) to study these systems is the UV-Vis spectrum, which is modulated both by conformational changes of the biliverdin chromophore and specific interactions with the apoprotein. In this work we present a theoretical study of the microscopic determinants of the UV-Vis spectrum of these compounds through the use of hybrid QM(TD-DFT)/MM techniques and molecular dynamics simulations. Comparing our results with existing experimental data, we prove that it is possible to predict spectroscopic properties, such as relative position and intensity ratio of main bands, with affordable methods, and to provide a microscopic explanation of them. This systematic information can be very useful for the study of described biliproteins or for those yet unknown.

We sought to investigate whether SARS-CoV-2 was present, and to perform full-length genomic sequencing, in a 5-year-old male crossbreed dog that presented with flu-like symptoms (including a dry hacking cough and mild dyspnea) and resided in a household with 3 adults that were diagnosed with SARS CoV-2 infection. Next generation sequencing based on MinION technology was performed on amplicons that were generated using a reverse transcriptase real-time polymerase chain reaction (RT-qPCR) of confirmed positive SARS-CoV-2 nasopharyngeal and buccal swabs, as well as a bronchoalveolar lavage with mean qCt value of 36 based on the Nucleocapsid gene. Descriptive comparisons to known sequences in Botswana and internationally were made using mutation profiling analysis and phylogenetic inferences based on maximum likelihood. Samples from the dog’s owners were not available. A near-full length SARS-CoV-2 genome (~90% coverage) was successfully genotyped and classified under clade 20 O and Pango-Lineage AY.43 (Pango v.4.0.6 PLEARN-v1.3; 2022-04-21), which is a sub-lineage of the Delta variant of concern (VOC) (formerly called B.1.617.2, first detected in India). We did not identify novel mutations that may be used to distinguish SARS-CoV-2 isolates from the dog and humans. In addition to S region mutation profiling, we performed phylogenetic analysis using Delta sequences from Botswana (n=1303); expectedly, the sequence isolated from the dog was closely related to the Delta sequences, particularly the AY.43, AY.116, and B.1.617.2 sub-lineages that were reported in Botswana within the same time frame. This is the first documented report of human-associated SARS-CoV-2 infection in a dog in Botswana. Although the direction of transmission remains unknown, this study further affirms the need for monitoring pets during different COVID-19 waves for possible clinically relevant SARS-CoV-2 transmissions between species.

Purpose: Peripartum cardiomyopathy (PPCM) is a rare complication of pregnancy manifesting with acute cardiac failure. Coronavirus Disease 2019 (COVID-19) can be complicated by cardiomyopathy, which can be difficult to differentiate from PPCM. The aim of this publication is to investigate different outcomes of cases with cardiomyopathy during pregnancy. Methods: Here, we report 4 peripartum cases with COVID-19 and acute cardiac failure due to cardiomyopathy. Moreover, to find previous similar cases, a comprehensive review on PPCM and COVID-19 associated cardiomyopathy was conducted in PubMed, Scopus, Embase, and Google Scholar. Results: Out of 4 cases we had, two cases deceased and one became a candidate for heart transplant. Despite advanced diagnostic approaches, differentiating COVID-19 associated cardiomyopathy from PPCM was challenging. According to the literature review, 7 cases with similar conditions were found. Conclusion: Pregnant women with COVID-19 may develop acute cardiac failure associated with PPCM or COVID-19 associated cardiomyopathy or both. This condition may be deadly due to rapid disease progression, atypical manifestations, and limited treatment options.

Objective: To assess the utility of a test-based approach to shorten isolation of healthcare workers with COVID-19 in the setting of the highly transmissible omicron variant Methods: Between December 24th, 2021, to January 5th, 2022 HCWs who tested positive for SARS-CoV-2 were re-tested at least five days since onset of symptoms. Results: 46 sequential fully COVID-19 vaccinated HCWs who had tested positive for SARS-CoV-2 underwent follow up testing. All the isolates were confirmed as omicron variants and only 4 (8.7%) were negative 5 days or more since onset of symptoms., Conclusions: Implementation of a test-based strategy is logistically challenging, increases costs and did not lead to shorter isolation in our institution.

Background. The recently emerged SARS-CoV-2 Omicron variant exhibits several mutations on the spike protein, enabling it to escape the immunity elicited by natural infection or vaccines. Avidity is the strength of binding between an antibody and its specific epitope. The SARS-CoV-2 spike protein binds to its cellular receptor with high affinity, and is the primary target of neutralizing antibodies. Therefore, protective antibodies should show high avidity. This study aimed at investigating the avidity of receptor-binding domain (RBD) binding antibodies and their neutralizing activity against the Omicron variant in COVID-19 patients and vaccinees. Methods. Samples collected from COVID-19 patients and from subjects who received homologous or heterologous vaccination were tested for the avidity of RBD-binding IgG and neutralizing antibodies against the wild-type SARS-CoV-2 virus and the Omicron variant. Results. In patients, RBD-binding IgG titres against the wild-type virus increased with time, but remained low. High neutralizing titres against the wild-type virus were not matched by high avidity or neutralizing activity against the Omicron variant. Vaccinees showed higher avidity than patients. Two vaccine doses elicited the production of neutralizing antibodies, but low avidity for the wild-type virus; antibody levels against the Omicron variant were even lower. Conversely, 3 doses of vaccine elicited high avidity and high neutralizing antibodies against both the wild-type virus and the Omicron variant. Conclusions. Repeated vaccination increases antibody avidity against the spike protein of the Omicron variant, suggesting that antibodies with high avidity and high neutralizing potential increase cross-protection against variants that carry several mutations on the RBD.

Anti-glutamic acid decarboxylase(Anti-GAD) are associated with various neurologic condition; but, no meningitis has been reported with it, so far. Evidence demonstrates the associated of autoimmune meningoencephalitis with Covid-19 infection. Here we report a 44-year-old female with progressive loss of consciousness with anti-GAD65 meningoencephalitis after Covid-19 infection.

SARS-CoV-2 is a virus that originated in Wuhan, China, in 2019 and has since spread worldwide, infecting people and animals. The objective of this study was to implement the plaque reduction neutralization test (PRNT90) and microneutralization (MN) to evaluate the presence of antibodies against SARS-CoV-2 in companion animals. First, a virus was isolated and sequenced, which showed a similarity of 99.9% with the original Wuhan strain. This virus had a titer of 3.55 DICC 50; for the PRNT90, it was adjusted to 30-40 plaque-forming units (PFU) and for the MN, at 100 DICC 50. For both assays, serial double dilutions of the sera were performed from 1:10 to 1:5120. Serum samples from people with negative and positive status were used as controls, as well as samples from companion animals (dogs and cats). In the case of human samples, the titers were from 1:10 to 1:5120 with PRNT and from 1:10 to 1:1280 with MN (r 2 = 0.957). Analysis of animal samples (dogs n = 521 and cats n = 29) revealed 10 positive samples, six from dogs and four from cats. Cats had higher titers than dogs. Titers ranged from 1:80 to 1:320 by MN and from 1:160 to 1:640 by PRNT90 for cats and from 1:10 to 1:40 by MN and from 1:10 to 1:80 PRNT90 for dogs (r 2 = 0.621). In both human and animal samples, the highest titers were observed with the PRNT90 test; however, the MN test is easier to perform than the PRNT90 test, so it is suggested as the test of choice for animal seroprevalence studies.

Background Household studies are crucial for understanding the transmission of SARS-CoV-2 infection, which may be underestimated from PCR testing of respiratory samples alone. We aim to combine assessment of household mitigation measures; nasopharyngeal, saliva and stool PCR testing; along with mucosal and systemic SARS-CoV-2 specific antibodies, to comprehensively characterise SARS-CoV-2 infection and transmission in households. Methods Between March and September 2020, we obtained samples from 92 participants in 26 households in Melbourne, Australia, in a 4-week period following onset of infection with ancestral SARS-CoV-2 variants. Results The secondary attack rate was 36% (24/66) when using nasopharyngeal swab (NPS) PCR positivity alone. However, when respiratory and non-respiratory samples were combined with antibody responses in blood and saliva, the secondary attack rate was 76% (50/66). SARS-CoV-2 viral load of the index case and household isolation measures were key factors that determine secondary transmission. In 27% (7/26) of households, all family members tested positive by NPS for SARS-CoV-2 and were characterised by lower respiratory Ct-values than low transmission families (Median 22.62 vs 32.91; IQR 17.06 to 28.67 vs 30.37 to 34.24). High transmission families were associated with enhanced plasma antibody responses to multiple SARS-CoV-2 antigens and the presence of neutralising antibodies. Three distinguishing saliva SARS-CoV-2 antibody features were identified according to age (IgA1 to Spike 1, IgA1 to nucleocapsid protein (NP), suggesting that adults and children generate distinct mucosal antibody responses during the acute phase of infection. Conclusion Utilising respiratory and non-respiratory PCR testing, along with measurement of SARS-CoV-2 specific local and systemic antibodies, provides a more accurate assessment of infection within households and highlights some of the immunological differences in response between children and adults.

Introduction: the role of vaccines in preventing diseases is well-established. However, the evolving techniques and types of vaccine necessitate the search for its safety more than ever. While cases of takotsubo cardiomyopathy and COVID-19 vaccine have been described, a comprehensive study to investigate the role of vaccination with cardiomyopathy and heart failure is lacking. The aim of this study is to investigate the link between the current vaccines and heart failure. Method: we utilized vaccine adverse event reporting system (VAERS) to search for cardiomyopathy, cardiac failure or ventricular dysfunction. Disproportionality signal analysis was conducted by measuring reporting odds ratio (ROR) with 95% confidence interval (CI). Results: VAERS reported over 1,300,000 adverse events between 1990-2021. Heart failure was reported over 2000 times in association with multiple vaccines. 56% of vaccine-related heart failure reported in males; 88% were serious; 19% mortality rate. The majority of reported heart failure was related to COVID-19 vaccines with ROR of 21.6 (CI: 18.3 to 25.4, P<0.0001). Smallpox was also significant with ROR 7.2 (CI 5.5-9.6, P<0.0001) On the other hand, heart failure was minimally described to be associated with other vaccines (influenza, zoster, tetanus, human papillomavirus (PPV)). Conclusions: Our study showed for the first time that vaccines maybe associated with heart failure. In particular, we show that covid-19 and smallpox vaccines are associated with increased risk of heart failure. Vaccines against Influenza, zoster, tetanus and (PPV) are associated with reduced heart failure. These data warrant longitudinal studies to delineate the association between heart failure and vaccines.

Since December 2019, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been spreading worldwide, triggering one of the most challenging pandemics in human population. In the light of reporting of this virus in domestic and wild animals from several parts of world, a systematic surveillance study was conceptualized to detect the SARS-CoV-2 among species of Veterinary importance. Nasal and/or rectal samples of 413 animals (Dog=195, cattle=64, horse=42, goat=41, buffalo=39, sheep=19, cat=6, camel=6 and monkey=1) were collected from different places of Gujarat state of India. RNA was extracted from samples and subjected to RT-qPCR based amplification of target sequences in viral nucleoprotein (N), spike (S) and ORF1ab genes. A total of 95 (23.79 %) animals were found positive, comprised of 67 (34.35 %) dogs, 15(23.43 %) cattle and 13(33.33 %) buffaloes. Overall, nasal samples (N=80/412, 19.41 %) gave more positive results than rectal samples (N=70/407, 17.19 %) in RT-qPCR. The whole SRAS-CoV-2 genome sequencing was done on one sample (ID-A4N; from dog) where 32 mutations, including 29 single nucleotide variation (SNV) and two deletions, were detected. Among them, 9 mutations were located in the receptor binding domain of the spike (S) protein. The consequent changes in amino acid sequence revealed that T19R, G142D, E156-, F157-, A222V, L452R, T478K, D614G, P681R mutation in S protein and D63G, R203M and D377Y in N protein. The lineage assigned to this SARS-CoV-1 sequence is B.1.617.2. Thus, the present study highlights the importance of SARS-CoV-2 surveillance in non-human host.

A document by Luise Erpenbeck, written on Authorea.

Objective. To compare the estimates of preterm birth (PTB; 22-36 weeks gestational age, GA) and stillbirth rates during COVID-19 pandemic in Italy with those recorded in the three previous years. Design. A population-based cohort study of liveborn and stillborn infants was conducted using data from Regional Health Systems and comparing the pandemic period (March 1st, 2020-March 31st, 2021, N= 362,129) to an historical period (January 2017- February 2020, N=1,117,172). The cohort covered 84.3% of the births in Italy. Methods. Logistic regressions were run in each Region and meta-analyses were performed centrally. We used an interrupted time series regression analysis to study the trend of preterm births from 2017 to 2021. Main Outcome Measures. The primary outcomes were PTB and stillbirths. Secondary outcomes were late PTB (32-36 weeks’ GA), very PTB (<32 weeks’ GA), and extreme PTB (<28 weeks’ GA), overall and stratified into singleton and multiples. Results. The pandemic period compared with the historical one was associated with a reduced risk for PTB (Odds Ratio: 0.90; 95% Confidence Interval, CI: 0.87, 0.93), late PTB (0.91; 0.87, 0.94), very PTB (0.87; 0.84, 0.91), and extreme PTB (0.88; 0.82, 0.94). In multiples, point estimates were not very different, but had wider CIs. No association was found for stillbirths (1.01; 0.90, 1.13). A linear decreasing trend in PTB rate was present in the historical period, with a further reduction after the lockdown. Conclusions We demonstrated a decrease in PTB rate after the introduction of COVID-19 restriction measures, without an increase in stillbirths.

We present a case series of 5 patients who were found to have COVID-19 encephalitis. There is still no disease defining test for diagnosis so the mainstay of diagnosis is the exclusion of all common causes of encephalitis. Brain MRI and CSF analysis perform an ancillary in the diagnostic tools.

During the COVID-19 pandemic, multiple skin manifestations have been described. These include an urticarial rash, morbilliform rash, maculopapular rash, vascular lesions, and varicella-like eruptions. A 30-year-old woman presented with a mild cough, then hives and pruritic rash for 3 days, followed by fever, dyspepsia, and throat pain for one day.

After a suspected allergic reactions to first dose of mRNA COVID-19 vaccine, given the PEG skin tests negativity and tolerance in vivo to PEG containing drugs, five patients were vaccinated with the second dose of Pfizer-Biontech undergoing a fractional protocol, with antihistamine premedication, without presenting immediate or delayed reactions.

The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak from December 2019 causing millions of deaths all over the world and the lack of specific treatment for severe forms of coronavirus disease 2019 (COVID-19) has led to vaccines development in record time with emergency use authorization in several countries increasing the risk of vaccine safety issues. Recently, several cases of Thrombotic Thrombocytopenic Purpura (TTP) have been reported following COVID-19 vaccination. TTP represents a life-threatening consumptive coagulopathy requiring urgent diagnosis and prompt treatment. It is a rare disease characterized by thrombocytopenia, microangiopathic hemolytic anemia and ischemic end-organ lesions. It can be either congenital or acquired. Various events such viral infections, medication, pregnancy, malignancies, and vaccinations may cause TTP. Clinicians should consider this diagnosis when evaluating thrombocytopenia in the post-vaccine period. Here, we report two cases of acquired TTP following Sinopharm COVID-19 vaccine (BBIBP-CorV) and Sinovac COVID-19 vaccine (CoronaVac). Diagnosis was based on clinical presentation and confirmed with severe reduction in the activity of von Willebrand factor-cleaving protease ADAMTS-13 and the presence of inhibitory autoantibodies. The two patients were successfully treated with corticosteroids, plasma exchange therapy and rituximab in the acute phase. In the literature, the reported cases of TTP induced by COVID-19 vaccination occurred after Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based COVID-19 Vaccines. To the best of our knowledge, this is the first report of acquired TTP after inactivated virus COVID-19 vaccines. A short literature review regarding acquired TTP patients following COVID-19 vaccines is also included.

Coronavirus disease-19 (COVID-19) is an emerging global pandemic. Since its emergence, the COVID-19-associated cutaneous manifestations have been increasingly reported, and they are extremely polymorphic.We report a case of acute generalized exanthematous pustulosis (AGEP) developed in a Tunisian adult, a few days after recovery from severe COVID-19 infection

A growing number of studies indicate a broad range of neurological manifestations, including seizures, occur in patients with COVID-19 infection. We report a 29-yers old female with status epilepticus and positive SARS-CoV-2 in the cerebrospinal fluid. Our findings support previous reports suggesting seizure as a possible symptom of COVID-19 infection.