<div>Bioassay guided isolation also have some pitfalls. Firstly, there is a high chance of discovering a compound that has already been discovered. Dereplication is near impossible. Although different species are being studied in this proposal, because they are related by genus there is a chance they will produce identical metabolites for a said biological activity. In addition, bioassays can be costly and time-consuming. In an ideal world, time would be an issue however there is a time limit of one year for this thesis. As mentioned previously, cultivation of marine sponges often results in low yields of compounds of interest. Samples sent away for bioassay are non-retrievable and causes an issue seeing as there is not overwhelming amount of sponges at our disposal. Although some assays only require 1 mg of material, that is sometimes all you get from isolation. There is also the issues of choosing your bioassays. Screening, for example, for antibiotic activity will exclude compounds with other important bioactivities. 
<br></div>