Ross Jones edited Lab Basics.tex  over 9 years ago

Commit id: e6fde09b605a7edb65e6b3ae02fdca2644d773d8

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\subsection{Craftable Materials}  \textbf{tubes}  \begin{itemize}  \item  PCR Tubes - for DNA amplification \item  1.5 mL tubes - for DNA / protein samples \item  50 mL tubes - for larger samples? \end{itemize}  \textbf{plates}  \begin{itemize}  \item  Cell culture plates (10 cm) - for growing cells/bacteria \item  96 well plates - for doing experiments \end{itemize}  \textbf{glassware}  \begin{itemize}  \item  Erlin-Meyer Flask (1L) - for growing bacteria \item  slides \item  beakers (1L) \end{itemize}  \textbf{solutions}  \begin{itemize}  \item  Bacterial Culture Medium ("LB"?) \item  Cell Culture medium ("DMEM"?) \item  Agar (can be crafted w/ LB to make LB agar --> crafted w/ cell culture plate to make bacterial culture plate) \item  Agarose? (for DNA / protein gels) - would anyone even want to use these? \item  Cell lysis solution \end{itemize}  \textbf{cell components}  \begin{itemize}  \item  plasmids \item  primers? \item  Cell DNA extract \item  (m)(si)RNA? \item  protein (+ antibodies?) \end{itemize}  \subsection{Craftable Equiment}  \textbf{tools}  \begin{itemize}  \item  micropipetter \item  pipetter \end{itemize}  \textbf{machines}  Machines will be built around plastic chassis and circuits which will be the standard units to build things by. Some will be more or less expensive and may span multiple blocks (see coke oven, steamer for multi-block examples in mods)  \begin{itemize}  \item  liquid nitrogen tank (cells) \item  freezer (cell components) \item  fridge? (some solutions) \item  centrifuge (+ ultra-centrifuge?) \item  PCR machine \item  microscope (add fluorescent filters to make fluorescent) \item  flow cytometer? (crafted with fluorescent microscope + expensive stuff) \end{itemize}