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Ross Jones edited Lab Basics.tex
over 9 years ago
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\subsection{Craftable Materials}
\textbf{tubes}
\begin{itemize}
\item PCR Tubes - for DNA amplification
\item 1.5 mL tubes - for DNA / protein samples
\item 50 mL tubes - for larger samples?
\end{itemize}
\textbf{plates}
\begin{itemize}
\item Cell culture plates (10 cm) - for growing cells/bacteria
\item 96 well plates - for doing experiments
\end{itemize}
\textbf{glassware}
\begin{itemize}
\item Erlin-Meyer Flask (1L) - for growing bacteria
\item slides
\item beakers (1L)
\end{itemize}
\textbf{solutions}
\begin{itemize}
\item Bacterial Culture Medium ("LB"?)
\item Cell Culture medium ("DMEM"?)
\item Agar (can be crafted w/ LB to make LB agar --> crafted w/ cell culture plate to make bacterial culture plate)
\item Agarose? (for DNA / protein gels) - would anyone even want to use these?
\item Cell lysis solution
\end{itemize}
\textbf{cell components}
\begin{itemize}
\item plasmids
\item primers?
\item Cell DNA extract
\item (m)(si)RNA?
\item protein (+ antibodies?)
\end{itemize}
\subsection{Craftable Equiment}
\textbf{tools}
\begin{itemize}
\item micropipetter
\item pipetter
\end{itemize}
\textbf{machines}
Machines will be built around plastic chassis and circuits which will be the standard units to build things by. Some will be more or less expensive and may span multiple blocks (see coke oven, steamer for multi-block examples in mods)
\begin{itemize}
\item liquid nitrogen tank (cells)
\item freezer (cell components)
\item fridge? (some solutions)
\item centrifuge (+ ultra-centrifuge?)
\item PCR machine
\item microscope (add fluorescent filters to make fluorescent)
\item flow cytometer? (crafted with fluorescent microscope + expensive stuff)
\end{itemize}