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\section{Conclusions}  \label{sec:conclusions}  FRETBursts is an open source and openly developed (see SI~\ref{sec:dev})  implementation of established smFRET burst analysis methods made available to the single-molecule community.  It implements several novel concepts which improve the analysis results, such as  time-dependent background estimation, background-dependent burst search threshold, 
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for timestamps and burst manipulation, making it an ideal platform for  developing and comparing new analytical techniques.  We envision FRETBursts both as a state-of-the-art burst analysis  software as well as a platform for development and assessment of novel algorithms.  To underpin this envisioned role, FRETBursts is developed following modern  
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to reduce over-engineering. Furthermore, to minimize the number software errors,  we employ defensive programming~\cite{Prli__2012} which includes code readability,  unit and regression testing and continuous integration~\cite{Eglen_2016}.  Finally, being open source, any individual can inspect the source code,  fix errors, adapt it to its own needs.  We believe that, in the single-molecule community,  standard open source software implementations, such as FRETBursts, can enhance