Antonino Ingargiola Use \namef for SI references  about 8 years ago

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contains links to the various resources. Installation instructions can be found in the  Reference Documentation (\href{http://fretbursts.readthedocs.org/en/latest/getting_started.html}{link}).  A description of FRETBursts execution using Jupyter notebooks is reported  in~\ref{sec:notebook}. in~\nameref{sec:notebook}.  % SI_link Detailed information on development style, testing strategies and  contributions guidelines are reported in~\ref{sec:dev}. in~\nameref{sec:dev}.  % SI_link Finally, to facilitate evaluation and comparison with other software,  we set up an on-line services allowing to execute FRETBursts  without requiring any installation on the user's computer (\href{https://github.com/tritemio/FRETBursts_notebooks#run-online}{link}). 

so that the bursts with largest sizes will have the largest weights.  Using size as weights (instead of any other monotonically increasing function  of size) can be justified noticing that the variance of bursts proximity ratio (PR) is  inversely proportional to the burst size (see~\ref{sec:burstweights_theory} (see~\nameref{sec:burstweights_theory}  for details). % SI_link In general, a weighting scheme is used for building efficient estimators for a population  parameter (e.g. the population FRET efficiency $E_p$). 

as possible to reduce statistical noise.  As a practical example, by fixing the burst size threshold to a low value (e.g. 10-20 photons)  and using weights, is possible to build a FRET histogram with well-defined FRET sub-populations peaks  without the need of searching an optimal burst-size threshold (\ref{sec:burstweights_theory}). (\nameref{sec:burstweights_theory}).  \paragraph{Python details}  FRETBursts has the option to weight bursts using $\gamma$-corrected 

\textit{Background estimation} section of the μs-ALEX tutorial  (\href{http://nbviewer.jupyter.org/github/tritemio/FRETBursts_notebooks/blob/master/notebooks/FRETBursts%20-%20us-ALEX%20smFRET%20burst%20analysis.ipynb#Background-estimation}{link}).  Finally, it is possible to use a slower but rigorous approach for finding the optimal  threshold as described in~\ref{sec:bg_opt_th}. in~\nameref{sec:bg_opt_th}.  % SI_link FRETBursts provides two kinds of plots to represent the background. One shows the histograms  of inter-photon delays compared to the fitted exponential distribution, shown in 

\end{lstlisting}  This command reflects the general form of plotting commands in FRETBursts  as described in~\ref{sec:plotting}. in~\nameref{sec:plotting}.  % SI_link Here we only note that the argument \verb|period| is an integer specifying the background  period to be plotted (when omitted, the default is 0, i.e. the first period).  Figure~\ref{fig:bg_dist_all} allows to quickly identify pathological cases where the 

\section{Conclusions}  \label{sec:conclusions}  FRETBursts is an open source and openly developed (see~\ref{sec:dev}) (see~\nameref{sec:dev})  implementation % SI_link of established smFRET burst analysis methods  made available to the single-molecule community.  It implements several novel concepts which improve the analysis results, such as