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single-molecule Förster Resonance Energy Transfer (smFRET) data. The software can analyze smFRET measurements  with one or several excitation spots~\cite{Ingargiola_2013}. The supported   excitation schemes include single lasers, Alternating Laser EXcitation (ALEX) with either two CW lasers (µs-ALEX \cite{Kapanidis_2005})   or two interleaved pulsed lasers (ns-ALEX \cite{16287971} or PIE Pulsed-Interleaved Excitation (PIE)  \cite{M_ller_2005}). FRETBursts is hosted and and openly developed~\cite{Prli__2012} on GitHub, where  issues, feature requests and contributions can be reported.