Antonino Ingargiola Address Reviewer 3, point 6.  almost 8 years ago

Commit id: e573fce9a686f70f59c14b27ed618e2317e8e37c

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in order to avoid under-representing some FRET sub-populations  due to different quantum yields of donor and acceptor dyes and/or  different photon detection efficiencies of donor and acceptor channels.  An alternative method to apply the $\gamma$ correction is to randomly  discard a fixed fraction of photons from either the DexDem or DexAem  photon stream as proposed by~\cite{tsukanov_detailed_2013}. This   simple and elegant method transforms the measurement data in order to  achieve $\gamma=1$, overcoming issues of selection bias between populations.   The only drawback of this method is that, by discarding a fraction of photons,  it leads to information loss and therefore to potentially decreased sensitivity   and/or accuracy.  A simple way to mitigate the dependence of the FRET distribution on   the burst size selection threshold is weighting bursts proportionally to their size          

in order to avoid under-representing some FRET sub-populations  due to different quantum yields of donor and acceptor dyes and/or  different photon detection efficiencies of donor and acceptor channels.  An alternative method to apply the $\gamma$ correction is to randomly  discard a fixed fraction of photons from either the DexDem or DexAem  photon stream as proposed by~\cite{tsukanov_detailed_2013}. This   simple and elegant method transforms the measurement data in order to  achieve $\gamma=1$, overcoming issues of selection bias between populations.   The only drawback of this method is that, by discarding a fraction of photons,  it leads to information loss and therefore to potentially decreased sensitivity   and/or accuracy.  A simple way to mitigate the dependence of the FRET distribution on  the burst size selection threshold is weighting bursts proportionally to their size