Antonio edited untitled.tex  over 9 years ago

Commit id: e26dedbb1d4b0bdb39307219adf2edbc9a81e06d

deletions | additions      

       

\textit{Oh, an empty article!} \section{FRETBursts: Burst analysis software for confocal smFRET experiments}  \author{Antonino Ingargiola}  You can get started by \textbf{double clicking} this text block Single-molecule FRET experiments allows to probe the distance between two fluorescent labels (\textit{donor}  and begin editing. You can also click \textit{acceptor}) on  the \textbf{Insert} button below 3-10nm range. In the commonly employed confocal geometry, molecules are free  to add new block elements. Or you diffuse in a solution. When a molecule crosse the excitation volume it emits a burst of photons that  can \textbf{drag be detected by single-photon detectors (SPADs). In a nutshell, assessing the relative intesity of the donor  and drop acceptor signal in each bursts it allows to infer their distance. smFRET experiments permit to study binding-unbunding processs or conformational changes of biomolecules and have proven to be  an image} right onto this text. Happy writing! unvaluable tool in studying fundamental cellular processes \cite{Kapanidis_2006}.  The concentration must be low enough that only one molecule a time will cross the extitation region. When a molecule crosses and a single or multiple excitation volumensFRETBursts is an opensource python package for data analysys of single-molecule FRET experiments in confocal geometry. FRETBursts can analyze.