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FRETBursts is an open source python software for burst analysis of confocal   single-molecule Förster Resonance Energy Transfer (smFRET) data. The software can analyze smFRET measurements  with one or several excitation spots~\cite{Ingargiola_2013}. The supported   excitation schemes include single lasers, Alternating Laser EXcitation (ALEX) with either  twoalternating  CW lasers (µs-ALEX\cite{Kapanidis_2005}) and or  two interleaved pulsed lasers (ns-ALEX or PIE\cite{M_ller_2005}). FRETBursts is hosted and and openly developed~\cite{Prli__2012} on GitHub, where  issues, feature requests and contributions can be reported.