Antonino Ingargiola edited Introduction.tex  almost 9 years ago

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of probing molecules and processes without perturbation from surface immobilization~\cite{Dahan_1999}\cite{Eggeling_1998}.   The field of freely-diffusing smFRET data analysis, has seen a number of significant   contributions over the years~\cite{Fries_1998}\cite{Eggeling_2001}\cite{Zhang_2005}\cite{Gopich_2005}\cite{Lee_2005}\cite{Nir_2006}\cite{Antonik2006}\cite{Gopich_2007}\cite{Gopich_2008}\cite{Camley_2009}\cite{Santoso_2010}\cite{Torella_2011}\cite{Tomov_2012}. However, so far, except for the some  fundamental steps of burst analysis, there is no single approach that is universally accepted and broadly applied.   On one hand this situation stems from the fact that  different approaches tend to answer slightly different questions.