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\subsection{smFRET and burst analysis}  FRETBursts is an open source python software for burst analysis of confocal   single-molecule FRET Förster Resonance Energy Transfer  (smFRET) data. The software can analyze smFRET measurements with one or several excitation spots~\cite{Ingargiola_2013}. The supported   excitation schemes include single lasers, two alternating CW lasers (µs-ALEX\cite{Kapanidis_2005})   and two interleaved pulsed lasers (ns-ALEX or PIE\cite{M_ller_2005}).