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\subsection{Open Science and Reproducibility}
In Over the
last past 20 years, single molecule FRET
(smFRET), (smFRET) has
emerged as grown into one of the most
useful techniques in single-molecule spectroscopy~\cite{Weiss_1999,Hohlbein_2014}.
Except a few specific While it is possible to extract information on subpopulations using ensemble
time-resolved measurements~\cite{Lerner_2014,Rahamim_2015}, measurements (e.g. ~\cite{Lerner_2014,Rahamim_2015}),
smFRET unique feature is
the its ability to
very straightforwardly resolve conformational
changes of biomolecules or measure binding-unbinding kinetics
on in heterogeneous samples.
smFRET measurements on freely diffusing molecules (the focus of this paper) have the
additional advantage
over measurements performed on immobilized molecules, of
probing allowing to probe molecules and processes without
possible perturbation from surface
immobilization~\cite{Dahan_1999,Eggeling_1998}. immobilization or additional functionalization needed for surface attachment~\cite{Dahan_1999,Eggeling_1998}.
The
field increasing amount of
work using freely-diffusing smFRET
data analysis, has
seen resulted in a
growing number of
significant theoretical contributions
over to the
years~\cite{Fries_1998,Eggeling_2001,Zhang_2005,Gopich_2005,Lee_2005,Nir_2006,Antonik2006,Gopich_2007,Gopich_2008,Camley_2009,Santoso_2010,Torella_2011,Tomov_2012}.
Historically, each specific topic of data analysis~\cite{Fries_1998,Eggeling_2001,Zhang_2005,Gopich_2005,Lee_2005,Nir_2006,Antonik2006,Gopich_2007,Gopich_2008,Camley_2009,Santoso_2010,Torella_2011,Tomov_2012}. Despite this profusion of methods, most research
group have implemented its groups still rely on their own
private version implementation of
analysis software (oftentimes highly dependent on a
particular setup
configuration) limited number of methods, with
almost no very little collaboration or code sharing.
Even in To clarify this statement, let us point that our
group, own group's past smFRET papers merely mention the
usage use of custom-made software without
additional details~\cite{Lee_2005,Nir_2006}.
This situation makes Even though some of this software tools are made available upon request, or shared publicly on websites (whether as source code or executable), it
remains hard to reproduce and validate
and to build upon results from different
groups.
Moreover, groups, let alone build upon them.
Additionally, as new methods are proposed in literature,
it is
oftentimes hard generally difficult to quantify their
performances. performance compared to other methods.
An independent quantitative assessment
would require a complete reimplementation, an effort
a few groups can afford.
As a results, potentially useful analysis improvements
are
either rarely
or slowly adopted by the
community at large. community.
In contrast with other
consolidated established traditions such as
sharing protocols and samples,
for in the domain of scientific software,
we have relegated ourselves to islands of non-communication.
From a more general stance, non-availability of codes