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Antonino Ingargiola edited Introduction.tex
about 8 years ago
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...
(section~\ref{sec:concepts}).
In section~\ref{sec:analysis}, we illustrate the steps involved
in smFRET burst analysis: (i) data loading (section~\ref{sec:dataload}),
)ii) (ii) definition of the
excitation alternation periods (section~\ref{sec:alternation}), (iii) background
correction (section~\ref{sec:bg_calc}), (iv) burst search (section~\ref{sec:burstsearch}),
(v) burst selection (section~\ref{sec:burstsel}) and (vi) FRET histogram fitting (section~\ref{sec:fretfit}).
The aim of this section is to illustrate the specificities and trade-off involved in various approaches
with sufficient details to enable readers new to the field (or Jupyter Notebooks)
to customize the analysis for their own needs.
Section~\ref{sec:bva} walks the reader thorough implementing
Burst Variance Analysis (BVA)~\cite{Torella_2011}, as an example of implementation
of an advanced data processing technique.
Finally, section~\ref{sec:conclusions} summarizes what we believe to be
the strengths of FRETBursts software.
...
links to relevant sections of documentation and other web resources
are displayed as ``(link)''.
In order to make the text more legible,
we have concentrated
python-specific Python-specific details in subsections entitled
\textit{Python details}. These subsections provide deeper insights for readers
already familiar with
python Python and can be initially skipped by readers who are not.
Finally, note that all commands here reported can be found in the
accompanying notebooks
(\href{https://github.com/tritemio/fretbursts_paper}{link}).