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Antonino Ingargiola edited Introduction.tex
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In the last 20 years, single molecule FRET (smFRET), has emerged as one of the most
useful techniques in single-molecule spectroscopy~\cite{Weiss_1999,Hohlbein_2014}.
Except
for a few
specific ensemble advanced time-resolved
measurements~\cite{Lerner_2014,Rahamim_2015}, techniques~\cite{Lerner_2014,Rahamim_2015},
smFRET unique
feature is the ability
to resolve is resolving conformational
changes of biomolecules or
measure measuring binding-unbinding kinetics on heterogeneous samples.
smFRET measurements on freely diffusing molecules (the focus of this paper) have the advantage
of probing molecules and processes without
possible perturbation from surface
immobilization~\cite{Dahan_1999,Eggeling_1998}.
The field of freely-diffusing smFRET data analysis, has seen a number of significant