Antonino Ingargiola Address Reviewer 3, point 6. (2)  almost 8 years ago

Commit id: a600711a133ffcffd378840644c8c1066cb99e25

deletions | additions      

       

due to different quantum yields of donor and acceptor dyes and/or  different photon detection efficiencies of donor and acceptor channels.  An alternative method to apply the $\gamma$ correction is to randomly  discard a fixed constant  fraction of photons chosen randomly  from either the DexDem or DexAem photon stream as proposed by~\cite{tsukanov_detailed_2013}. stream~\cite{Nir_2006}.  This simple and elegant method transforms the measurement data in order to  achieve $\gamma=1$, overcoming issues the issue  of selection bias between populations. This approach has also the advantage of preserving  the binomial distribution of D and A photons in each burst, so that peaks  of FRET populations are easier to model statistically.  The only drawbackof this method  is that, by discarding a fraction of photons, it this method  leads to information loss and therefore to potentially decreased a potential   decrease in  sensitivity and/or accuracy. A simple way to mitigate the dependence of the FRET distribution on          

due to different quantum yields of donor and acceptor dyes and/or  different photon detection efficiencies of donor and acceptor channels.  An alternative method to apply the $\gamma$ correction is to randomly  discard a fixed constant  fraction of photons chosen randomly  from either the DexDem or DexAem photon stream as proposed by~\cite{tsukanov_detailed_2013}. stream~\cite{Nir_2006}.  This simple and elegant method transforms the measurement data in order to  achieve $\gamma=1$, overcoming issues the issue  of selection bias between populations. This approach has also the advantage of preserving  the binomial distribution of D and A photons in each burst, so that peaks  of FRET populations are easier to model statistically.  The only drawbackof this method  is that, by discarding a fraction of photons, it this method  leads to information loss and therefore to potentially decreased a potential   decrease in  sensitivity and/or accuracy. A simple way to mitigate the dependence of the FRET distribution on  the burst size selection threshold is weighting bursts proportionally to their size