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Antonino Ingargiola edited Burst_Weights_Theory.tex
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\subsubsection{Experiments}
Figure X show a comparison of a FRET histogram obtained from the same burst
with and without weights. The burst selection is obtained applying a burst size
of threshold of
20 counts, 10 counts (after background correction), in order to filter
the extreme low-end of the burst size
distribution which include background bursts. distribution.
The use of size-weighted FRET histograms
allows is a simple way to obtain a
minimal with representation of
the various FRET
peaks without distribution that is optimal (in the sense of not discarding information) while removing
the need of using a manually adjusted high threshold for burst selection.
While an increase in the selection threshold
reduces the shot-noise of the residual population it also discards the useful
information of low and medium size bursts. Necessarly, an unweighted histograms
with high selection threshold will exhibit an higher statistical noise
(due to the reduced number of bursts) compared to the size-weighted histogram
obtained using a low threshold.
statistical variance