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Antonino Ingargiola edited Introduction.tex
almost 9 years ago
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\subsection{What is FRETBursts}
In the last 20 years, single molecule FRET (smFRET), has emerged as one of the most
useful techniques in single-molecule
spectroscopy~\cite{Weiss_1999}. spectroscopy~\cite{Weiss_1999},\cite{Hohlbein_2014}.
As opposed to ensemble experiments, smFRET allows to resolve conformational
changes of biomolecules or measure binding-unbinding kinetics on heterogeneous samples.
smFRET measurements on freely diffusing molecules (the focus of this paper) have the advantage