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Antonino Ingargiola edited Concepts.tex
about 9 years ago
Commit id: 8e98b0b894ae8b2175357eed15577ec88763f963
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\label{sec:ph_streams}
The fundamental data at the core of smFRET experiments is the array of photon
arrival timestamps, with a temporal resolution
usually of the order of 10~ns. In single-spot
measurements, all the timestamps are stored in a single array. In multi-spot
measurements~\cite{Ingargiola_2013} we have as many timestamps arrays as the number of excitation
spots.
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An estimation of the background rates is needed both to select a proper threshold for
burst search and to correct the raw burst counts by subtracting the background counts.
Furthermore, it is advisable to
periodially periodically estimate the background
in order to track occasional variations during the measurement. Experimentally
we found that when the background is not constant, it varies on time scales of tens of seconds
(see figure~\ref{fig:bg_timetrace}). FRETBursts splits the data in uniform time
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\href{http://fretbursts.readthedocs.org/en/latest/plots.html}{built-in plot functions}
for \verb|Data| objects.
The plot syntax is the same both for single and multi-spot measurements.
Almost all the The majority of plot commands are called through the wrapper function
\verb|dplot|, for example to plot a timetrace of the photon data we type:
\begin{verbatim}