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Antonino Ingargiola edited Concepts.tex
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section~\ref{sec:burstsel}.
\subsection{Burst
sizes and weights}
As will be shown in the following sections, the The number of photon detected during a burst
is commonly called the ``burst size''.
Bursts sizes are usually computed using all photons (e.g. \verb|nd + na + naa|),
or photons detected during a single excitation period (e.g. \verb|nd + na|).
Burst search
returns bursts yields a distribution of
different burst sizes
(i.e. number of photons). that approximately
follows an exponential distribution.
Bursts with large size
(that indeed (which contain most of the information)
are much less frequent than bursts with smaller sizes. For this reason,
in order
to resolve FRET populations and accurately assess their mean FRET efficiency, it is
of
paramount importance important to select
bursts with size burst sizes larger than a
given threshold
(see in order
to properly characterize FRET populations(see section~\ref{sec:burstsel}).
The
choice of burst size threshold is
critical because critical.
A too low threshold will
for instance widen broaden the FRET
histogram populations and introduce
artifacts (spurious peaks
owing to and patterns) because for the
inclusion majority of
small sized bursts
having higher FRET uncertainty. E and S are ratios of small integers.
Conversely
a too high threshold will result in a lower number of bursts
and
FRET estimation
can suffer from possibly poor
statistics. statistics in representing FRET populations.
In order to mitigate the dependence on the burst size threshold it may be
useful to weight bursts according to their size (i.e. their