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Eitan Lerner edited Introduction.tex
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immobilization or additional functionalization needed for surface attachment~\cite{Dahan_1999,Eggeling_1998}.
The increasing amount of work using freely-diffusing smFRET has resulted in a growing number of theoretical contributions to the specific topic of data analysis~\cite{Fries_1998,Eggeling_2001,Zhang_2005,Gopich_2005,Lee_2005,Nir_2006,Antonik2006,Gopich_2007,Gopich_2008,Camley_2009,Santoso_2010,Torella_2011,Tomov_2012}. Despite this profusion of methods, most research groups still rely on their own implementation of a limited number of methods, with very little collaboration or code sharing. To clarify this statement, let us point that our own group's past smFRET papers merely mention the use of custom-made software without
additional details~\cite{Lee_2005,Nir_2006}. Even though some of
this these software tools are made available upon request, or shared publicly on websites (whether as source code or executable), it remains hard to reproduce and validate results from different groups, let alone build upon them.
Additionally, as new methods are proposed in literature,
it is generally difficult to quantify their performance compared to other methods.
An independent quantitative assessment