Antonino Ingargiola edited Concepts.tex  about 8 years ago

Commit id: 71c8f85b4e1fb2a90df749a5b0091b2c1098d483

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For this reason we encourage performing a burst size selection after background  correction, possibly taking into account the γ factor, as discussed in  sections~\ref{sec:burstsizeweights} and~\ref{sec:burstsel}.  In special situations, users can also choose to replace this size selection  with another criterion such as burst duration or brightness.  \subsection{γ-corrected Burst Sizes and Weights}  \label{sec:burstsizeweights}  The number of photons detected during a burst, commonly called ``burst size'',  is usually computed using either all photons, or photons detected   during donor excitation period. To compute the burst size $n_t$, size,  FRETBursts uses one of the following formulas:  \begin{equation}  \label{eq:burstsize_dex}  n_t n_{dex}  = n_a + \gamma\,n_d \end{equation}  \begin{equation} 

\noindent where $n_d$, $n_a$ and $n_{aa}$ are, similarly to the attributes   in tables~\ref{tab:data_n}, the background-corrected  burst counts in different channels and excitation periods,  and $\gamma$ is periods.  $\gamma$, dubbed  the γ factor. ``γ factor'', takes into account  different quantum yield of donor and acceptor dyes and different  photon detection efficiencies between donor and acceptor detection  channel~\cite{Lee_2005}.  Eq.~\ref{eq:burstsize_dex} includes only photons during donor excitation periods,   while eq.~\ref{eq:burstsize_allph} includes all photons.  Burst sizes computed according to eq.~\ref{eq:burstsize_dex}