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\section{Implementing Burst Variance Analysis}  In this section we describe how to implement the Burst Variance Analysis (BVA)~\cite{Torella_2011},  as an example of easily building (BVA)~\cite{Torella_2011}.  FRETBurts provides well-tested, general-purpose functions for timestamps and burst data   manipulation and therefore simplifies implementing  custom burst analysis algorithms leveraging the core manipulation   functions provided by FRETBursts. such as BVA.  Single-molecule FRET histograms show more information than just mean FRET efficiencies.   While, in general, several peaks indicate the presence of multiple subpopulations,