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\subsection{Installing FRETBursts}  \label{sec:install}  Installing  FRETBursts is a analogous to installing any standard  python package that requires package. A python environment needs to be installed, preferentially  through  astandard set of  scientific python libraries, included by distribution which conveniently include  all the major scientific python   distributions.   Specifically, numerical computations are performed with numpy/scipy,   plotting is based on matplotlib and seaborn, a few core functions are  optimized with cython and the software is preferentially run through  a Jupyther (IPython) Notebook. libraries.  For new python users we suggest installing   \href{https://store.continuum.io/cshop/anaconda/}{Continuum Anaconda}  python distribution. distribution and then install (or update)  FRETBurstscan be installed through the standard python package manager (PIP)  or through \href{http://conda.pydata.org/docs/}{conda}.  Alternatively the latest development version can be downloaded directly   from GitHub. For more information on different installation methods refer to the   \href{http://fretbursts.readthedocs.org/en/latest/getting_started.html}{Getting Started}  section in the FRETBursts documentation.  \subsection{Executing FRETBursts}  \label{sec:import}  In general, we suggest to import FRETbursts  with the expression: command:  \begin{verbatim}  >>> import conda install  fretbursts as fb -c tritemio  \end{verbatim}  after which all For more information on different installation methods refer to  the FRETBursts functions will be availabe under \href{http://fretbursts.readthedocs.org/en/latest/getting_started.html}{Getting Started}  section in  the\verb|fb.|  prefix. In this article, for brevity, we assume that  FRETBursts is imported   with the shortcut form instead:  \begin{verbatim}  >>> from fretbursts import *  \end{verbatim} documentation.  that allows skipping the \verb|fb.| prefix and it will also import some common   numeric libraries (i.e. \textit{numpy} FRETBursts depends on a standard set of scientific   python libraries, namely, numpy/scipy for numerical computations,   matplotlib  and \textit{matplotlib.pyplot}   imported as \verb|np| seaborn for plotting. Optionally, a few core functions are  optimized with cython  and \verb|plt| respectively). the software is preferentially run through  a Jupyther (IPython) Notebook (optional).  For a complete list of dependencies can be found in the   \href{http://fretbursts.readthedocs.org/en/latest/getting_started.html}{Getting Started}  section in the FRETBursts documentation.  In order \subsection{Running FRETBursts}  The preferential way  to enable the reproducibility of the analysis and to ease  the sharing execute FRETBurts is by running one  of thecomplete workflow we encourage using  FRETBursts through   the IPython Notebook environment.   IPython notebooks tutorials which  aredocuments  inwhich  the code is interspersed with   prose in form of Jypyther notebooks.  Jupyther (formerly IPython) notebooks are documents,   accessed through  a web-based format web browser,  thatcan  contain math formulas, hyperlinks both code  and figures (or anything else a web browser rich text (including formulas, hyperlinks, figures, etc...).  FRETBursts tutorials are notebook that  can display).  Furthermore, the be re-executed,  modified or used to process new data files with minimal modifications.  The  "notebook workflow"\cite{Shen_2014}has the advantage   of automatically recording  not only eases  theall  analysis commands and parameters comprehension (by integrating the cone in a rich document)  but also the data file names, greatly enhance its reproducibility by saving an execution trail  including  software versions versions, input files, parameters, commands  and all  the full output   (figures, analysis results (text, figures,  tables, etc...) in a single document that can be interactively   modified and re-executed. etc...).  All Given  the FRETBursts tutorials are   ipython notebooks and, in fact, modality of execution, running FRETBurst does not require  any prior python knowledge. The user only needs familiarize with  the preferred way notebook graphical environment  to start a new analysis is by   copying be able to navigate  and modifying a pre-existing FRETBursts notebook. run the notebooks.