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Antonino Ingargiola edited Background estimation.tex
over 9 years ago
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\subsection{Background estimation}
\label{sec:bg_calc}
The first step of smFRET analysis involves estimating the background level.
As noted in section~\ref{sec:bg_intro}, estimating the background rate involves estimating the rate of a Poisson process.
To For example, to compute the background every 30s, using a threshold of 2 ms for all the photon streams we execute:
\begin{verbatim}
d.calc_bg(bg.exp_fit, time_s=30, tail_min_us=2000)
\end{verbatim}
Here, the first argument is the actual fitting function used to fit the
background in each period and for each photon
stream. stream~\ref{sec:bg_intro}. The function
\verb|bg.exp_fit| fits the background using a Maximum Likelihood Estimation
(MLE) of the delays distribution. More fitting functions are available in
\verb|bg| namespace (see the