Antonino Ingargiola edited burst-search.tex  about 8 years ago

Commit id: 4c941b0429dae947a2a24205d2b16209537ed402

deletions | additions      

       

\begin{lstlisting}  d.gamma = 0.85  d.leakage = 0.04 0.15  d.dir_ex = 0.08  \end{lstlisting} 

These correction factors can be used to display corrected FRET distributions.  However, when the goal is to fit the FRET efficiency of sub-populations,   it is more accurate simpler  to fit the uncorrected FRET histogram (i.e. background-corrected proximity ratio) and then correct the fitted FRET efficiency (see SI in~\cite{Lee_2005}, SI).  This procedure Correctiong the PR after fitting (instead of correcting the full burst data)  avoids distortion of the FRET distributions due to the corrections, distribution  and keeps peaks of static FRET subpopulations closer to the ideal Binomial statistics~\cite{Gopich_2007}.  FRETBursts implements the correction formulas for $E$ and $S$ in the functions