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Antonino Ingargiola edited burst-selection.tex
about 8 years ago
Commit id: 2254652e576dc4ddecb86c02732c46af9d5f675a
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\noindent and the resulting selection is plotted in figure~\ref{fig:alex_jointplot}.
Another important parameter for defining the burst size is the
$\gamma$-factor, γ-factor, i.e.
the imbalance between the donor and the acceptor channels. As noted in
section~\ref{sec:burstsizeweights}, the
$\gamma$-factor γ-factor is
used to compensate bias for the different fluorescence quantum yields of the D and A
fluorophores as well as the different photon-detection efficiencies of the D and A channels.
When
gamma-factor γ-factor is not 1, neglecting its effect on burst size leads to
over-representing (in terms of number of bursts) one population versus to the others.
When the
gamma γ factor is known, users can pass the argument
\verb|gamma| during burst selection:
\begin{lstlisting}
...
\href{http://fretbursts.readthedocs.org/en/latest/burst_selection.html#fretbursts.select\_bursts.size}{\texttt{select\_bursts.size} documentation}.
Furthermore the \textit{Data} class provides the
\href{http://fretbursts.readthedocs.org/en/latest/data_class.html#fretbursts.burstlib.Data.burst_sizes}{method \texttt{Data.burst\_sizes()}} that returns the
gamma-corrected γ-corrected burst sizes with or
without the addition of \verb|naa|.
\subsubsection{Select the FRET Populations}