Antonino Ingargiola edited burst-selection.tex  about 8 years ago

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\noindent and the resulting selection is plotted in figure~\ref{fig:alex_jointplot}.  Another important parameter for defining the burst size is the $\gamma$-factor, γ-factor,  i.e. the imbalance between the donor and the acceptor channels. As noted in  section~\ref{sec:burstsizeweights}, the $\gamma$-factor γ-factor  is used to compensate bias for the different fluorescence quantum yields of the D and A  fluorophores as well as the different photon-detection efficiencies of the D and A channels.  When gamma-factor γ-factor  is not 1, neglecting its effect on burst size leads to over-representing (in terms of number of bursts) one population versus to the others.  When the gamma γ  factor is known, users can pass the argument \verb|gamma| during burst selection:  \begin{lstlisting} 

\href{http://fretbursts.readthedocs.org/en/latest/burst_selection.html#fretbursts.select\_bursts.size}{\texttt{select\_bursts.size} documentation}.   Furthermore the \textit{Data} class provides the  \href{http://fretbursts.readthedocs.org/en/latest/data_class.html#fretbursts.burstlib.Data.burst_sizes}{method \texttt{Data.burst\_sizes()}} that returns the gamma-corrected γ-corrected  burst sizes with or without the addition of \verb|naa|.  \subsubsection{Select the FRET Populations}