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can then be related to the distance between the two dyes.  The analysis of smFRET experiments involves identifying photon bursts from   single-molecules in a continuous stream of photon, photons,  estimating the background and other correction factors, filtering and finally extracting the corrected   FRET efficiencies for each sub-population in the sample. In this paper we introduce FRETBursts,  a software for confocal smFRET data analysis, which