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Jenna M. Lang edited Materials and Methods.tex
about 8 years ago
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\section{Methods}
Triplicate leaf and root samples were taken from one individual of each distinct species found at each site. These were stored at room temperature in
the Zymo Xpedition stabilization
and lysis buffer
in a sterile tube until DNA extraction.
DNA was extracted The MoBio PowerSoil from the samples and used as template in PCR amplification of the V3-V4 region of the 16S SSU rRNA gene. Samples were cleaned and pooled to 1 ng/µL DNA concentration and sequenced on an Illumina MiSeq at the UC Davis Genome Center Sequencing Core. Sequences were demultiplexed using a custom in-house script. Fasta files were then analyzed using the
standard QIIME workflow for analyzing microbial community
data. data (an Ipython notebook detailing the scripts used for analysis is included in the data submission on Figshare. Figures and statistics were produced in R using the phyloseq package.