Jenna M. Lang edited Materials and Methods.tex  about 8 years ago

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\section{Methods}  Triplicate leaf and root samples were taken from one individual of each distinct species found at each site. These were stored at room temperature in the Zymo Xpedition  stabilization and lysis  bufferin a sterile tube  until DNA extraction. DNA was extracted The MoBio PowerSoil  from the samples and used as template in PCR amplification of the V3-V4 region of the 16S SSU rRNA gene. Samples were cleaned and pooled to 1 ng/µL DNA concentration and sequenced on an Illumina MiSeq at the UC Davis Genome Center Sequencing Core. Sequences were demultiplexed using a custom in-house script. Fasta files were then analyzed using thestandard  QIIME workflow for analyzing microbial community data. data (an Ipython notebook detailing the scripts used for analysis is included in the data submission on Figshare.  Figures and statistics were produced in R using the phyloseq package.