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\section{Initial Findings}  \subsection{Alpha Diversity}  Observed OTUs, Shannon diversity index, and Simpson's diversity index were used to calculate statistics for alpha diversity. Within leaves, the p-value was less than 0.05 between sites P2 and P3, P2 and P4, as well as P2 and P1 using observed OTUs and the Shannon diversity index. Within roots, the p-value was less than 0.05 between sites P1 and P4, and P2 and P1 when using Shannon; the p-value was less than 0.05 between sites P2 and P3, and P2 and P1 using Simpson's diversity index. Root communities also had a p-value less than 0.05 between Redhead Grass and Vallisneria using all three diversity measures.  Microbes from the genera Methylotenera, Planctomyces, Rhodobacter, and Providencia are the most common amongst all the sites and SAV species. Site P1 specifically had a higher relative abundance of the genus Prosthecobacter and family Verucomicrobiaceae than the other sites. In addition, site P2 had a high relative abundance of Cylindrospermopsis in the leaves of Eurasian watermilfoil and Vallisneria. The genera Sulfuricurvum and Sulfurimonas, which are sulfur-oxidizing bacteria, were present in the roots at site P2 and were particularly high in relative abundance in Redhead Grass. Rhodocyclaceae was also commonly present in root samples from sites P2 and P3.  \subsection{Beta Diversity}  Fig. 2 shows a PCoA using unweighted UniFrac. Panel  A shows the whole dataset. Leaves and roots tend to cluster separately within each site. In addition, the sites cluster into two groups: P1, P3, P4 make up one group and P2 makes up another group. group (p-value = 0.001).  Panel B shows data for only site P2 in order to examine patterns between samples within only one site. Site P2 was chosen since it was the most distinct in microbial community compared to P1, P3, and P4. It is apparent that samples are differentiated by whether they are leaves or roots and by the host species. species (p-value = 0.001).  Leaves and roots are most differentiated in Redhead Grass and Vallisneria. At the same time, the leaves (regardless of host) tend to cluster more tightly with each other than the roots with themselves. Still, there are distinct clusters differentiated by the host species within site P2. P2 (p-value = 0.001).  Unfortunately, since salinity readings were taken at varying tide heights, the salinity gradient was not apparent in this dataset. In addition, only four sites, all in same end of the river, were collected from. This was a failing of the experimental design. However, the water and sediment chemistry at each site was unique, and PCoA plots revealed that most of these parameters show the same or similar clustering as in Figure 2A.