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\section{Future Directions}  Based on these preliminary findings, it is clear that more research is needed to elucidate the relationship between SAV growing along the Potomac and their microbiomes. Since this project's sites were determined based on an unrelated study's travel plans, An unforeseen downside of coupling our collections to those of another study is that  SAV were only found in four of the twelve sites visited. With better planning, SAV can be sampled all along the Potomac River, as well as the James River, which provides a near-replicate salinity gradient.Adding to this data set would also increase the statistical weight of the future findings and perhaps bring to light new relationships that we cannot see in our limited dataset.  In addition, we found that a future study of this system would require salinity measurements during the same tide height for consistency. This data would help find a stronger correlation, if one exists, to changes in the SAV microbiome. As of now, our consistency across sites. Here,  salinity measurements were taken at different tide heights and are therefore inconsistent with historical data that shows the salinity gradient along the Potomac River. These findings indicate that there may be an interesting pattern among the water and sediment chemistry at each site that drives the microbial community. Further research could focus on disentangling which changes are due to host species vs. location factors biogeochemical parameters  vs. tissue type, as well as which microbes are key players in driving those shifts.