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# Subjects  Eight male _A. burtoni_ with an average length of 49.6 +- 2.2 mm and weight of 3.4 +- .37 grams were used in this study. They were housed individually with three females and allowed to become Dominant for at least two weeks prior to the study. Animals were killed by rapid decapitation, brains were extracted immedieatly, placed in O.C.T and frozen on dry ice. # Sectioning  The brains were randomly split into two groups of four. One group was sectioned at 16uM while the other was sectioned at 30uM. All brains were sectioned in four series, series:  three of the four were used for COX histochemistry at varying incubation times (30, 60, 90 min) and the fourth series of each individual was used for a Nissl stain. Slides were processed in two batches and slides were removed from the batch in turn at each time point. Each time point included 1-2 slides of brain homogenate sectioned at 10, 20, 30, 40, 50 and 60uM as in internal standard curve. In addition three slides of mouse brain sectioned at 40uM (donated by the Crews lab) were included as positive control and processed at the 60 min time point.  # COX histochemistry  # Nissl  # Quantification