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**Fig 3.**  Docked model of pNPG in the active site of BglB showing native residues (yellow) and mutated residues (purple) for (A) N404C, (B) W120F, (C) H178A, and (D) E222H. Effect of temperature on purified BglB displayed on the right. Activity was measured on an eight row gradient, ranging from 30- 50 C.