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Bill Chan edited Results_Protein_production_and_baseline__.md
about 8 years ago
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[Summarize protein production specifics]
## Thermal stability of mutants compared to wild type
The melting temperature determined for wild type was 39.6
degrees C. The average melting temperature across all
specific point mutations was 40.7 degrees C (plus or minus .7
degrees C).
This The overall effect of point mutations was
roughly the same as minimal on melting temperature, suggesting that
point mutations generally do not affect the protein structure. However, at certain residues, these point mutations have a large effect on the stability of
wild type's. the protein. The melting temperature range for the mutants was 33.8 degrees C to 45.3 degrees
C, a deviation of six degrees in either direction from that of wild type. Mutation N404C increased the protein's stability as it displayed a melting temperature of 45.3 C.
Specific mutations resulted Conversely, mutation H178A displayed a substantial decrease in
protein stability with a melting temperature
that deviated from that of
wild type's by roughly six 33.8 degrees
in either direction. C. [Describe what we found regarding the range of thermal stabilities we observed]
## Mutants have [effect] on thermal stability
Mutation N404C increased in its stability as it displayed a melting temperature of 45.3 degrees C. Mutation H178A was less thermostable compared to wild type, displaying a melting temperature of 33.8 degrees C.
[Describe what we found here]