Bill Chan edited Methods.md  about 8 years ago

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## Data analysis   Rates of 4NP formation at each of 8 temperatures from 30 C to 50 C were fit to the logistic equation $$\textrm{rate} = \frac{1}{1+e^{-k*(x-x0)}}$$ to determine the Tm-activity of each protein. Proteins with a calculated Tm outside of our range of detection were discarded, leaving 76 77  unique samples. ## Rosetta modeling of $\DeltaG$