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Bill Chan edited Results_Protein_production_and_baseline__.md
about 8 years ago
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# Results
## Protein production and baseline values
A total of 117 proteins, including native BglB, were purified with immobilized metal affinity chromatography and eluted in 200 μL HEPES buffer. Of the 117 proteins synthesized, 79 proteins express and purify as soluble protein.
69 of these mutants displayed a melting temperature fit to our logistic equation. Greater than 75% of mutants maintained the yields obtained by native BglB while 32% did not express and purify as a soluble protein above our limit of detection (0.1 mg/mL) for protein yield after purification based on A280 and SDS-PAGE.
Of the 79 proteins with expression, 69 mutants displayed a melting temperature. Native BglB displayed an average melting temperature of 39.6 C.
[Summarize protein production specifics]