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**Fig 3.** Superposition of mutated residues (purple) over native residues (yellow) for (A) N404C, (B) W120F, (C) H178A, and (D) E222H. Effect of temperature on purified BglB displayed on the right. Activity was measured on an eight row gradient, ranging from 30- 50 C. Image drawn with PyMOL.Melting curves drawn with ____.