Bill Chan edited Results_Protein_production_and_baseline__.md  about 8 years ago

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## Summary of effects of mutations on BglB thermal stability   The wild type BglB across three replicates had an average melting temperature of 39.6 C. Across 69 point mutations, the average melting temperature was 39.0 C within a range of 33.8 C and 45.3 C. The average kurtosis was -0.75. 58% of mutations were within one degree of the native BglB, consistent with rational design of the mutants for stability within a physically-realistic force field. 36% of mutations exhibited a higher melting temperature than that of native BglB. The mutant conferring the largest increase in Tm was found to be N404C, which increased the Tm of BglB to [degrees] 45.3  C, nearly six degrees relative to wild type. The mutation that decreased the thermal stability of BglB the most was H178A, which resulted in a decrease in Tm by 7 C. ## Mutants with increased thermal stability