Bill Chan edited Results_Protein_production_and_baseline__.md  about 8 years ago

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[Summarize protein production specifics]  ## Thermal stability of mutants compared to wild type   The melting temperature determined for wild type was 39.6 C. The average melting temperature across all point mutations was 40.7 40.1  degrees C (plus or minus .7 .88  C). The overall effect of point mutations was minimal on melting temperature, suggesting that point mutations generally do not affect the protein structure. However, at certain residues, these point mutations have a large effect on the stability of the protein. The melting temperature range for the mutants was 33.8 degrees C to 45.3 degrees C, a deviation of six degrees in either direction from that of wild type. Mutation N404C increased the protein's stability as it displayed a melting temperature of 45.3 C. Conversely, mutation H178A displayed a substantial decrease in protein stability with a melting temperature of 33.8 degrees C. [Describe what we found regarding the range of thermal stabilities we observed]