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Table of designed epoxide reductases incorporating a dyad catalytic motif and using a substrate walking approach
Scaffold Mutations BEO PO EO PR New Q8
2V6G K121G+F127I+N179V+M189A+G272E+F317A+I321H+C326F 0 0 0 0 0
2V6G K121G+N179T+M189A+G272E+F317A+I321H+C326F 0 0 0 0 0
2V6G K121G+N179V+M189A+G272E+F317A+I321H+C326F 0 0 0 0 0
2V6G K121H+N179T+M189A+G272E+F317A+I321H 0 0 0 0 0
2V6G K121H+N179T+M189A+G272E+F317A+I321W 0 0 0 0 0
2V6G K121W+M189A+G272E+F317A+I321H 0 0 0 0 0
2V6G K121W+M189A+G272E+F317A+I321Y 0 0 0 + 0
2V6G K121W+N179P+M189A+G272E+F317A+I321Y 0 0 0 0 0
2V6G K121W+N179T+M189A+G272E+F317A+I321H+C326F 0 0 0 0 0
2V6G K121W+N179V+M189A+G272E+F317A+I321H 0 0 0 + 0
1LC3 A11H+E88A+F242H 0 0 0 0 0
1LC3 A11H+E88A+F242W 0 0 0 + 0
1LC3 A11H+E88A+I241L+F242W 0 0 0 0 0
1LC3 A11H+E88A+M121W+F143W+S162W+F242W 0 0 0 0 0
1LC3 A11H+E88A+M121W+F242W 0 0 0 0 0
1LC3 A11H+E88A+M121W+S162W+F242W 0 0 0 0 0
1LC3 A11H+E88A+V117T+F242H 0 0 0 0 0
1LC3 A11H+E88A+V117T+F242W 0 0 0 0 0
1LC3 A11H+E88A+V117T+I241L+F242W 0 0 0 0 0
1LC3 A11H+E88G+F242H 0 0 0 + 0
3ORF C181W+R182A+L183F+P184F+W185M 0 0 0 + 0
3ORF C181W+R182A+P184F+W185M 0 0 0 0 0
3ORF C181W+R182A+P184R+W185M 0 0 0 0 0
3ORF C181W+R182A+W185M+L208P+L231M 0 0 0 0 0
3ORF C181W+R182G+P184R+W185R 0 0 0 0 0
3ORF F180I+C181W+R182A+W185M+L208P+L231M 0 0 0 + 0
3ORF F180I+W185M+L208P+Q224P+L231M 0 0 0 + 0
3ORF L208P+L231M 0 0 0 + 0
3ORF W185M+L231M 0 0 0 + 0
3JYO T18Y+D109H+A140Q+Q257S 0 0 0 0 1
3JYO T18Y+N93G+Q257S 0 0 0 0 1
3JYO T18Y+T68Q+K72M+N93A+D109N+Q257S 0 0 0 0 1
3JYO T18Y+K72S+D109W+G136E+Q257S 0 0 0 0 1
3JYO T18Y+D109H+V137N+A140Q+Q257S 0 0 0 0 1
3JYO T18Y+T68Q+D109N+Q257S 0 0 0 0 1
3JYO T18Y+T94R+Q257S 0 0 0 0 1
3JYO L8Y+T18Y+D109W+Q257S 0 0 0 0 1
3JYO T18Y+T68Q+N93G+D109N+Q257S 0 0 0 0 1
3JYO T18Y+D109W+G136D+Q257S 0 0 0 0 1
3JYO L8F+T18Y+D109W+Q257S 0 0 0 0 1
3JYO T18Y+T68Q+N93A+D109N+Q257S 0 0 0 0 1
3JYO T18Y+N93A+D109H+Q257S 0 0 0 0 1
3JYO T18Y+Q257S 0 0 0 0 1
3JYO T18Y+D109W+Q257S 0 0 0 0 1
3JYO T18Y+N93A+D109N+Q257S 0 0 0 0 1
3JYO T18Y+D109H+Q257S 0 0 0 0 1
3JYO T18Y+T68Q+K72M+D109N+Q257S 0 0 0 0 1
3JYO T18Y+N66A+T94R+Q257S 0 0 0 0 1
1OAA A100F+V105A+Y168A+K172Y 0 0 0 0 1
1OAA A100S+L102H+V105A+Y168A+K172Y 0 0 0 0 1
1OAA A100S+L102K+V105A+Y168A+K172Y 0 0 0 0 1
1OAA A100S+Y168A+K172Y 0 0 0 0 1
1OAA A100Y+L102K+V105A+Y168A+K172Y 0 0 0 0 1
1OAA L102A+C157S+Y168H+K172S+M203W 0 0 0 0 1
1OAA L102A+C157S+Y168H+M203W 0 0 0 0 1
1OAA L102A+C157W+Y168H+M203W 0 0 0 0 1
1OAA L102A+S155Q+Y168H+M203W 0 0 0 0 1
1OAA L102A+V105H+S155A+Y168H+A195C+M203W+A207M 0 0 0 0 1
1OAA L102A+V105H+S155A+Y168H+A195C+M203W 0 0 0 0 1
1OAA L102A+V105H+S155A+Y168H+M203W 0 0 0 0 1
1OAA L102A+V105H+S155Q+Y168H+M203W 0 0 0 0 1
1OAA L102A+Y168H+M203W 0 0 0 0 1
1OAA L102G+V105G+Y168A+K172Y+N201D+Q204A+A207H 0 0 0 0 1
1OAA Y168A+K172Y+N201D+Q204A 0 0 0 0 1