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## Author contributions (alphabetical by last name)   + Dylan Alexander Carlin [2]: molecular cloning, wrote protocols, designed experiments, wrote software used in analysis, analyzed data, performed computational Rosetta modeling, FoldX  modeling,performed  machine learning, wrote paper + Ryan Caster [1]: characterized expression for mutants   + Bill Chan [1]: characterized Tm for mutants, analyzed data, contributed to paper   + Natalie Damrau [1]: characterized mutants   + Siena Hapig-Ward [1]: characterized Tm and kinetic constants, analyzed data, drew figures,  contributed to paper + Mary Riley [1]: characterized mutants   + Justin B. Siegel [1,3,4]: PI