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## Summary of effects of mutations on BglB thermal stability  The wild type BglB across three replicates had an average melting temperature of 39.6 C. Across 69 point mutations, the average melting temperature was 39.0 C within a range of 33.8 C and 45.3 C. The average kurtosis was -0.75. 58% of Tm values for mutants were within one degree of the native BglB value. Of the 114 mutants reported, 36% exhibited a lower melting temperature than that of native BglB and 9% displayed a higher melting temperature. The largest Tm observed in this data set is N404C, which increased the Tm of BglB to 45.3 C (dTm=+5.4 ˚C). The mutation that decreased the thermal stability of BglB the most was H178A (dTm=-7 C). (dTm=-7.0 ˚C).