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## Author contributions (alphabetical by last name)   + Dylan Alexander Carlin [2]: molecular cloning,wrote protocols,  designed experiments, wrote software used in analysis, analyzed data, Rosetta modeling, FoldX modeling, machine learning, wrote paper + Ryan Caster [1]: characterized expression for mutants   + Bill Chan [1]: characterized Tm for mutants, analyzed data, contributed to paper   + Natalie Damrau [1]: characterized mutants