Yen-Lin Chen edited section_Wet_SEM__.tex  over 8 years ago

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\section{Wet SEM}  From the previous discussions, even though the sample chamber can have high pressure of tens of Torr, the liquid evaporation from the sample could not be totally ignored. Due to the low pressure, the sample would still be dried out within several hours and the specimens were no longer maintained in the original environment of interest. For biological specimens, the cells shrink to some extent that the functioning of certain organ in the cells was disrupted. For inorganic materials, some chemical interactions require the solvent environment, such as electrolyte for batteries. Therefore, the partially hydrated sample for ESEM image is not appropriate for certain interest. The goal is to preserve $100\%$ of the liquid environment in the sample, which implies either that the pressure in the sample chamber is approximately $1atm$ or that the fully hydrated sample must be protected by coating or layered materials without compromising the spatial resolution.   The