deletions | additions       diff --git a/ESEM_provides_the_suitable_environment__.tex b/ESEM_provides_the_suitable_environment__.tex  index 627fe41..6f56171 100644  --- a/ESEM_provides_the_suitable_environment__.tex  +++ b/ESEM_provides_the_suitable_environment__.tex 
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The experiment was also done by imaging the gold coated specimens. Coating is the standard processing technique for conventional SEM but not for ESEM. Both the ESD images and the BSE images for the coated biological specimens (\textit{Spirogyra} and \textit{E. gracilis}) show that the topological and internal information is lost, Fig.2.e. The thickness of metal coating ranges from $0.1 \sim 10 \mu m$ and the SE cannot escape from even deeper. The large atomic number of gold contributes mainly to the BSE signals, therefore suppressing the signals from the cell itself.  This experiment demonstrated that the appropriate application on biological specimens and the difference between ESD and BSE imaging signals. It also showed that the metal coating would jeopardize the image and the preparation process of the samples could be minimized or ruled out.