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Yen-Lin Chen added ESEM_is_widely_used_in__.tex
over 8 years ago
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ESEM is widely used in biological specimens. The dynamics of the closure and open of stomatal pores was also investigated by ESEM image \cite{McGREGOR_2009}. The time scale of such process ranges from several minutes to hours due to the environment. Therefore, by controlling the water vapor pressure in the sample chamber, the time scale of the closure and open of stomatal pores could be modified. The ESEM was proved to be eligible for temporal resolution of several minutes depending upon the dwelling time of each pixel.
ESEM can also be combined with other characterization tools to have more powerful impact. Due to the high spatial resolution ($<1 \mu m$) and temporal resolution ($<30s$), one recent interesting research demonstrated that it's possible to slice a single yeast cell of the size $\approx 2 \mu m$ by atomic force microscopy(AFM) tips which were fabricated by focused ion beam(FIB) \cite{Shen_2015}. The ESEM can image the whole slicing process of yeast cells and the slicing angle and location can also be controlled precisely. In addition, this "nano-knife" can manipulate the yeast cells one by one within several seconds.
